Similar results were found in pp60c-src precipitated from AEV-transformed chicken and rat cells.
Partial proteolytic phosphopeptide analysis revealed a decrease in phosphorylation of both serine- and tyrosine-containing peptides, suggesting an activation of specific phosphatases or inhibition of specific kinases in the AEV-transformed quail cells.
#Butel arc 500 1.1 software#
A concomitant 50-80% reduction of 32Pi incorporation into the pp60c-src protein from radiolabeled, transformed cells was also observed, indicating a relationship between hypophosphorylation and diminished enzyme activity. Butel ARC500 scanner programming and control software for GRE PSR 600, PSR 500, RadioShack PRO-106 and RadioShack PRO-197 police scanner radios. Using an immune-complex kinase assay, we found that the specific activity of pp60c-src in AEV-transformed quail cells was decreased by a factor of 6-30 relative to that found in uninfected quail cells. When monitoring P25 digital systems, GRE's exclusive Automatic Adaptive Digital Tracking instantly adapts the digital decoder to the digital modulation format.
Talkgroup and individual call monitoring is supported. A continuous cell line from AEV-infected quail embryo fibroblasts was derived that exhibited a typical transformed phenotype and expressed the viral oncogene products, p75gag-erbA and gp66-68erbB. The PSR-500 scans most common trunked radio system signaling formats, including Motorola, EDACS, LTR and P25 trunked radio networks. Since tyrosine protein kinases have been implicated in the process of normal growth signal transduction, we wished to study the possible consequences of the expression of these mutated, growth-regulating receptor genes on the activity of the cellular tyrosine kinase pp60c-src. AEV encodes two oncogenes, v-erbA and v-erbB, whose products are closely related to the thyroxine receptor and the epidermal growth factor receptor, respectively.
Avian erythroblastosis virus (AEV) is a replication-defective retrovirus that causes erythroblastosis and sarcomas in chickens and transforms immature erythroid cells and fibroblasts in culture.